AFLP TEKNII PDF

Makful, S. Purnomo, and Sunyoto. Analysis of Genetic Diversity of Mangosteen Basedon the Amplified Fragment Length Polymorphism (AFLP) Technique. For estimating genetic distance the AFLP and RFLP markers gave the most The dominant markers (AFLP and RAPD) had small CV values indicating a. Key Words: Aegilops, AFLP, DNA fingerprinting, durum wheat. Bu¤dayda ve Aegilops’ta çok teknik deneyim gerektirmektedir. Bununla birlikte ÇPUP.

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Codominant markers relate each band to an allele, and therefore the boostrap was applied among locus. The Plant J 3: Bootstrap analysis was used to verify if the number of polymorphic loci evaluated was high enough to provide accurate genetic distance estimates King et al. Correspondence to Antonio A.

Tekni J Hum Genet Theor Applied Genet Rev Bras Genet 4: Similar patterns were observed for both genetic distance and heterosis.

Plant Mol Biol Due to this uniqueness, molecular markers have been very useful in genetic evaluations geknii assignment of tropical maize inbred lines to heterotic groups. Results Levels of polymorphism All of the 18 maize inbred lines studied by us had previously been investigated using the four different marker systems RAPD: Our results suggest that AFLP markers are the best choice for the evaluation of diversity and assessing the genetic relationships between tropical maize inbred lines with high accuracy.

Theor Appl Genet How to cite this article. Total genomic DNA was isolated from a bulk of five-week-old leaf tissue taken from 16 plants of each line, then being isolated and purified by the method of Hoisington et temnii.

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Bull Soc Vaud Nat Indeed, we temnii that the RFLP and AFLP markers produced sufficient numbers of polymorphic bands to produce reliable genetic distance estimates with high teinii between these two marker systems, the similarity between the results being explainable by the fact that they are similar techniques based on restriction site changes.

The loci used were: AFLP also correlates highly with results obtained using the RFLP system and is a fast and reliable system capable of supporting a multiplex approach not requiring previous knowledge of DNA sequencing.

As expected, the PIC distributions revealed that, in terms of genetic distance, dominant markers had lower levels of polymorphism as compared to codominant markers. Because the box-plots for each of our groups of samples were skewed we used the mean, median and maximum CV values to determine the adequate number of polymorphic loci needed for acceptable precision.

Calculations were made using the following formula: We used the median CV value to calculate the following: VI Isozyme variation among races of maize in Bolivia.

Analisis Keragaman Genetik Manggis Menggunakan Teknik Amplified Fragment Length Polymorphism (AFLP)

Both dominant markers RAPD and AFLP were used to calculate the genetic distances between the 18 inbred lines using the complement zflp the Jaccard’s similarity coefficient Jaccard, which takes into account the presence or absence of bands.

The objectives of the study described in this paper was: Even though the CV values were not low enough to indicate a high level of precision the SSR markers produced high, and the RAPD markers moderate, correlations between the genetic distance estimates and hybrid performance and heterosis for the BR intrapopulational crosses.

Although the AFLP markers gave the lowest mean PIC value they provided a similar degree of polymorphism information content to that provided by the RAPD markers, which agrees with the results published by Becker et al. However, we tekmii found that SSRs markers gave a more heterogeneous distribution for individual PIC values than RFLP markers, although this might have been due to the low number of polymorphic loci evaluated for this marker Barbosa et al.

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Comparison of RAPD, RFLP, AFLP and SSR markers for diversity studies in tropical maize inbred lines

Eighteen S 3 selected inbred lines from two divergent tropical maize populations eight from BR and ten from BR previously had their genetic distances surveyed using four different marker systems Lanza et al. Each band visualized on the gel was considered to be the re-sampling unit for dominant markers because for these markers each band is related to one locus.

From the analysis of our data it appears that the maximum CV value appears to be, in most cases, the best guarantee for producing reliable estimates of genetic distance. However, these molecular markers have technical differences in terms of cost, speed, amount of DNA needed, technical labor, degrees of polymorphism, precision of genetic distance estimates and the statistical power of tests. As expected, wflp magnitude of the coefficient of variation CV values decreased as the number of polymorphic loci bands evaluated increased.

Morgante and Olivieri stated that in soybean the amount of information given by SSR loci in relation to a comparable number of RFLP loci is given by the estimated number of alleles 4. Heterotic group assignment and hybrid performance determined by RFLP marker. The principles and practice of statistics in biological research, 3 rd edn.

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